MersV1, Main, Exploration, bibRecord, 002B84

TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell

Identifieur interne : 002B84 ( Main/Exploration ); précédent : 002B83; suivant : 002B85

TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell

Auteurs : Elise Chiffoleau [France] ; Jean-Marie Heslan [France] ; Michele Heslan [France] ; Cedric Louvet [France] ; Thomas Condamine [France] ; Maria-Cristina Cuturi [France]

Source :

International Immunology [ 0953-8178 ] ; 2007.

RBID : ISTEX:2BEC040FE30927A2B2DA362EC41F90DB524ADC19

Abstract

Toll-like receptors (TLRs) play a crucial role in the initiation of innate responses following microbial infection and also in adaptive immune responses by orchestrating the activation of different cell populations. TLRs are expressed at high levels in antigen-presenting cells and recent studies have demonstrated the expression and biological role of TLRs in mouse and human CD4+ T cells. In this study, we analyzed TLR mRNA expression in rat CD4+ T cells using stringent quantitative reverse transcription–PCR conditions enabling a direct comparison of the levels of each TLR. We show that TLR3, 5, 6 and 9 mRNAs are the highest TLRs expressed in rat CD4+ T cells and that TLR5 mRNA is highly expressed in regulatory CD4+ CD25+ T cells. In addition, we show that the TLR9 ligand (TLR9L), CpG oligodeoxynucleotide, synergizes with anti-CD3 to induce proliferation of both CD4+ CD25− and regulatory CD4+ CD25+ T cells and that TLR9L partially abrogates the suppressive activity mediated by regulatory CD4+ CD25+ T cells. This loss of suppression is in part due to the direct effect of TLR9L on effector T cells that are rendered more resistant to the regulation exerted by regulatory T cells. To our knowledge, this is the first study describing the expression of TLR mRNA in rat CD4+ T cells and the capacity of TLR9L to directly regulate rat T cell responses. Thus, TLR9L may rapidly increase the host's adaptive immunity by expanding effector cells and also by attenuating the suppressive activity mediated by regulatory T cells.

Url:

https://api.istex.fr/ark:/67375/HXZ-F1X9TKCG-T/fulltext.pdf

DOI: 10.1093/intimm/dxl136

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001530
to stream Istex, to step Curation: 001530
to stream Istex, to step Checkpoint: 000837
to stream Main, to step Merge: 002C10
to stream Main, to step Curation: 002B84

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell</title>
<author><name sortKey="Chiffoleau, Elise" sort="Chiffoleau, Elise" uniqKey="Chiffoleau E" first="Elise" last="Chiffoleau">Elise Chiffoleau</name>
</author>
<author><name sortKey="Heslan, Jean Marie" sort="Heslan, Jean Marie" uniqKey="Heslan J" first="Jean-Marie" last="Heslan">Jean-Marie Heslan</name>
</author>
<author><name sortKey="Heslan, Michele" sort="Heslan, Michele" uniqKey="Heslan M" first="Michele" last="Heslan">Michele Heslan</name>
</author>
<author><name sortKey="Louvet, Cedric" sort="Louvet, Cedric" uniqKey="Louvet C" first="Cedric" last="Louvet">Cedric Louvet</name>
</author>
<author><name sortKey="Condamine, Thomas" sort="Condamine, Thomas" uniqKey="Condamine T" first="Thomas" last="Condamine">Thomas Condamine</name>
</author>
<author><name sortKey="Cuturi, Maria Cristina" sort="Cuturi, Maria Cristina" uniqKey="Cuturi M" first="Maria-Cristina" last="Cuturi">Maria-Cristina Cuturi</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:2BEC040FE30927A2B2DA362EC41F90DB524ADC19</idno>
<date when="2007" year="2007">2007</date>
<idno type="doi">10.1093/intimm/dxl136</idno>
<idno type="url">https://api.istex.fr/ark:/67375/HXZ-F1X9TKCG-T/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001530</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001530</idno>
<idno type="wicri:Area/Istex/Curation">001530</idno>
<idno type="wicri:Area/Istex/Checkpoint">000837</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000837</idno>
<idno type="wicri:doubleKey">0953-8178:2007:Chiffoleau E:tlr:ligand:enhances</idno>
<idno type="wicri:Area/Main/Merge">002C10</idno>
<idno type="wicri:Area/Main/Curation">002B84</idno>
<idno type="wicri:Area/Main/Exploration">002B84</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">TLR9 ligand enhances proliferation of rat CD4<hi rend="superscript">+</hi>
 T cell and modulates suppressive activity mediated by CD4<hi rend="superscript">+</hi>
 CD25<hi rend="superscript">+</hi>
 T cell</title>
<author><name sortKey="Chiffoleau, Elise" sort="Chiffoleau, Elise" uniqKey="Chiffoleau E" first="Elise" last="Chiffoleau">Elise Chiffoleau</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Santé et de la Recherche Médicale Unité 643 and Institut de Transplantation Et de Recherche en Transplantation, CHU Hotel Dieu, 30 Boulevard Jean Monnet, 44093 Nantes Cedex 01</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">France</country>
</affiliation>
</author>
<author><name sortKey="Heslan, Jean Marie" sort="Heslan, Jean Marie" uniqKey="Heslan J" first="Jean-Marie" last="Heslan">Jean-Marie Heslan</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Santé et de la Recherche Médicale Unité 643 and Institut de Transplantation Et de Recherche en Transplantation, CHU Hotel Dieu, 30 Boulevard Jean Monnet, 44093 Nantes Cedex 01</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Heslan, Michele" sort="Heslan, Michele" uniqKey="Heslan M" first="Michele" last="Heslan">Michele Heslan</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Santé et de la Recherche Médicale Unité 643 and Institut de Transplantation Et de Recherche en Transplantation, CHU Hotel Dieu, 30 Boulevard Jean Monnet, 44093 Nantes Cedex 01</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Louvet, Cedric" sort="Louvet, Cedric" uniqKey="Louvet C" first="Cedric" last="Louvet">Cedric Louvet</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Santé et de la Recherche Médicale Unité 643 and Institut de Transplantation Et de Recherche en Transplantation, CHU Hotel Dieu, 30 Boulevard Jean Monnet, 44093 Nantes Cedex 01</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Condamine, Thomas" sort="Condamine, Thomas" uniqKey="Condamine T" first="Thomas" last="Condamine">Thomas Condamine</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Santé et de la Recherche Médicale Unité 643 and Institut de Transplantation Et de Recherche en Transplantation, CHU Hotel Dieu, 30 Boulevard Jean Monnet, 44093 Nantes Cedex 01</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cuturi, Maria Cristina" sort="Cuturi, Maria Cristina" uniqKey="Cuturi M" first="Maria-Cristina" last="Cuturi">Maria-Cristina Cuturi</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Santé et de la Recherche Médicale Unité 643 and Institut de Transplantation Et de Recherche en Transplantation, CHU Hotel Dieu, 30 Boulevard Jean Monnet, 44093 Nantes Cedex 01</wicri:regionArea>
<placeName><region type="region" nuts="2">Pays de la Loire</region>
<settlement type="city">Nantes</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">International Immunology</title>
<idno type="eISSN">1460-2377</idno>
<idno type="ISSN">0953-8178</idno>
<imprint><publisher>Oxford University Press</publisher>
<date>2007</date>
<date type="e-published">2007</date>
<biblScope unit="vol">19</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="193">193</biblScope>
<biblScope unit="page" to="201">201</biblScope>
</imprint>
<idno type="ISSN">0953-8178</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0953-8178</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Toll-like receptors (TLRs) play a crucial role in the initiation of innate responses following microbial infection and also in adaptive immune responses by orchestrating the activation of different cell populations. TLRs are expressed at high levels in antigen-presenting cells and recent studies have demonstrated the expression and biological role of TLRs in mouse and human CD4+ T cells. In this study, we analyzed TLR mRNA expression in rat CD4+ T cells using stringent quantitative reverse transcription–PCR conditions enabling a direct comparison of the levels of each TLR. We show that TLR3, 5, 6 and 9 mRNAs are the highest TLRs expressed in rat CD4+ T cells and that TLR5 mRNA is highly expressed in regulatory CD4+ CD25+ T cells. In addition, we show that the TLR9 ligand (TLR9L), CpG oligodeoxynucleotide, synergizes with anti-CD3 to induce proliferation of both CD4+ CD25− and regulatory CD4+ CD25+ T cells and that TLR9L partially abrogates the suppressive activity mediated by regulatory CD4+ CD25+ T cells. This loss of suppression is in part due to the direct effect of TLR9L on effector T cells that are rendered more resistant to the regulation exerted by regulatory T cells. To our knowledge, this is the first study describing the expression of TLR mRNA in rat CD4+ T cells and the capacity of TLR9L to directly regulate rat T cell responses. Thus, TLR9L may rapidly increase the host's adaptive immunity by expanding effector cells and also by attenuating the suppressive activity mediated by regulatory T cells.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Pays de la Loire</li>
</region>
<settlement><li>Nantes</li>
</settlement>
</list>
<tree><country name="France"><region name="Pays de la Loire"><name sortKey="Chiffoleau, Elise" sort="Chiffoleau, Elise" uniqKey="Chiffoleau E" first="Elise" last="Chiffoleau">Elise Chiffoleau</name>
</region>
<name sortKey="Chiffoleau, Elise" sort="Chiffoleau, Elise" uniqKey="Chiffoleau E" first="Elise" last="Chiffoleau">Elise Chiffoleau</name>
<name sortKey="Condamine, Thomas" sort="Condamine, Thomas" uniqKey="Condamine T" first="Thomas" last="Condamine">Thomas Condamine</name>
<name sortKey="Cuturi, Maria Cristina" sort="Cuturi, Maria Cristina" uniqKey="Cuturi M" first="Maria-Cristina" last="Cuturi">Maria-Cristina Cuturi</name>
<name sortKey="Heslan, Jean Marie" sort="Heslan, Jean Marie" uniqKey="Heslan J" first="Jean-Marie" last="Heslan">Jean-Marie Heslan</name>
<name sortKey="Heslan, Michele" sort="Heslan, Michele" uniqKey="Heslan M" first="Michele" last="Heslan">Michele Heslan</name>
<name sortKey="Louvet, Cedric" sort="Louvet, Cedric" uniqKey="Louvet C" first="Cedric" last="Louvet">Cedric Louvet</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B84 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B84 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:2BEC040FE30927A2B2DA362EC41F90DB524ADC19
   |texte=   TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell

TLR9 ligand enhances proliferation of rat CD4+ T cell and modulates suppressive activity mediated by CD4+ CD25+ T cell

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri